Nuclear Transplantation of Mouse Inner Cell Mass and Trophectoderm Cells into Enucleated Two-Cell Embryos.

Do trophectoderm and inner cell mass cells in the mouse blastocyst maintain discrete lineages?

The extent to which trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst remain distinct during the period from the commencement of cavitation up until 48 h later in culture was investigated. Fluorescent latex microparticles were used to label exclusively all TE cells in nascent blastocysts and the position of labelled progeny in cultured blastocysts was examined by dis...

The Effects of Ethanol and Strontium on Growth and Development of Two-Cell Arrested Mouse Embryos

Interaction between inner cell mass and trophectoderm of the mouse blastocyst. II. The fate of the polar trophectoderm.

Selective labelling of polar trophectoderm cells in early mouse blastocysts has allowed the fate of polar cells to be followed during in vitro and in vivo blastocyst development. Results show that there is cell movement from polar to mural regions as blastocysts grow. This indicates that trophectoderm cells directly opposite the inner cell mass are the oldest mural cells. However, after implant...

Lineage analysis of inner cell mass and trophectoderm using microsurgically reconstituted mouse blastocysts.

The fate of mouse blastocyst tissues was examined following reconstitution of blastocysts from isolated inner cell mass (ICM) and trophectoderm differing for electrophoretic variants at the glucose phosphate isomerase (GPI-1) locus. A modified microsurgical method was used and a more sensitive enzyme assay allowed finer dissection of developing chimaeric conceptuses. In seven of nine cases, the...

Paternal DNA strands segregate to both trophectoderm and inner cell mass of the developing mouse embryo.

The localization of sperm DNA strands was examined in preimplantation mouse embryos. Male mice were treated with bromodeoxyuridine (BrdU) to label germ-cell-line DNA and were then mated with unlabeled females. Sperm DNA strands in early embryos derived from these matings could be detected by means of a fluorescent antibody specific to BrdU. The position and number of fluorescent spots detected ...